Description
The ToupTek E3ISPM20000KPA Microscopy Camera is a high-resolution scientific imaging solution designed for professional microscopy applications requiring exceptional detail, color accuracy, and imaging performance. Equipped with a powerful 20MP Sony IMX183 CMOS sensor, this camera delivers ultra-sharp images with a maximum resolution of 5440 × 3648 pixels, making it ideal for laboratory research, industrial inspection, pathology, biological studies, and educational applications.
Featuring a large 1-inch back-illuminated sensor, the E3ISPM20000KPA provides excellent light sensitivity and improved signal-to-noise performance compared to smaller sensor cameras. This allows the camera to capture bright, detailed images even in challenging low-light conditions such as fluorescence microscopy or high-magnification observation. The advanced hardware ISP processing system further enhances image quality through accurate color reproduction, automatic white balance, exposure adjustment, and noise reduction.
The high-speed USB 3.0 interface ensures fast and stable data transmission for smooth live viewing and rapid image capture. Users can enjoy responsive real-time imaging while maintaining high resolution and image clarity. Flexible ROI (Region of Interest) functionality also allows faster frame rates when working with selected image areas.
Designed for versatility, the camera is compatible with a wide range of microscope systems including biological microscopes, metallurgical microscopes, stereo microscopes, and fluorescence microscopes. The standard C-mount design allows simple integration with many optical setups and adapters.
ToupTek includes professional imaging software with advanced functions such as measurement, image stitching, focus stacking, annotation, and video recording. The camera also supports multiple operating systems including Windows, macOS, Linux, and Android, making it suitable for both laboratory and industrial environments.
With its combination of high resolution, excellent sensitivity, robust construction, and professional software support, the ToupTek E3ISPM20000KPA is an excellent choice for users seeking reliable and high-quality digital microscopy imaging.
E3ISPM20000KPA is a digital camera specifically designed for microscopic imaging applications, featuring a IMX183 (C,RS) high-performance image sensor with the following characteristics:
- Exceptional Imaging Quality: 20M (5440×3648) resolution with 2.4 µm × 2.4 µm pixel size, sensor size of 13.06 mm × 8.76 mm , ensuring clear detail presentation
- Imaging Characteristics: Features Rolling Shutter design, supports color imaging, suitable for brightfield, darkfield, phase contrast, fluorescence and other microscopy observation methods
- High-Speed Data Transfer: Utilizes USB3.0 interface with maximum frame rate up to 15 fps@5440×3648; 50 fps@2736×1824; 60 fps@1824×1216 , supporting real-time preview and rapid capture
- Color Reproduction: 8/12-bit color depth with dynamic range reaching TBD , accurately reproducing sample colors
- Sensitivity Performance: Excellent low-light performance with sensitivity of 462 mV with 1/30s; 0.21 mV with 1/30s , suitable for low-light sample observation
- Optical Compatibility: Standard C-mount interface, compatible with various microscope brands, optional 0.5×/0.63×/1× adapters available
- Compact Design: Overall dimensions of only TBD , weighing TBD , powered by USB3.0 interface power (compatible with USB2.0) , no additional power supply required
- Software Support: Includes professional microscopy imaging software ToupView, supports Windows/Linux/macOS/Android multi-platform SDK (native C/C++, C#/VB.NET, Python, Java, DirectShow, TWAIN, etc.) systems, provides measurement, annotation, stitching, focus stacking and other functions, supports C/C++, C#, Python, ImageJ, μManager development environments
ToupTek E3ISPM20000KPA Microscopy Camera Specification:
| Key Parameters | |
| Model | E3ISPM20000KPA |
| Sensor | IMX183 (C, RS) |
| Effective Pixels / Resolution | 20M (5440×3648) |
| Frame Rate (Full Resolution) | 15 fps@5440×3648; 50 fps@2736×1824; 60 fps@1824×1216 |
| Shutter Type | Rolling Shutter |
| Color Type | Color |
| Imaging Performance | |
| Pixel Size | 2.4 µm × 2.4 µm |
| Sensor Size | 13.06 mm × 8.76 mm |
| Diagonal | 1" (15.73 mm) |
| Dynamic Range | TBD |
| Bit Depth | 8/12-bit |
| Sensitivity | 462 mV with 1/30s; 0.21 mV with 1/30s |
| Interface & Mechanical | |
| Data Interface | USB3.0 |
| GPIO | TBD |
| Lens Mount | C-mount |
| Dimensions | TBD |
| Weight | TBD |
| Power Supply | USB3.0 interface power (compatible with USB2.0) |
| Environment & Certification | |
| Operating Temperature / Humidity | -10 °C ~ +50 °C / 30%~80%RH |
| Storage Temperature / Humidity | -20 °C ~ +60 °C / 10%~60%RH |
| Operating System | Windows/Linux/macOS/Android multi-platform SDK (native C/C++, C#/VB.NET, Python, Java, DirectShow, TWAIN, etc.) |
| Certification | CE, FCC |
Penicillium W.M



U3ISPM Camera Dimensions
For more information, read the following article:
ToupTek E3ISPM20000KPA Review: A High-Resolution Camera for Modern Digital Microscopy







Package Contents
Standard configuration and packing details for E3ISPM series hardware ISP USB 3.0 CMOS cameras